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Formation of compact globular particles in interphase nuclei from rat liver under the effect of polyanionsстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 сентября 2013 г.
- Авторы: Prusov A.N., Fais D., Polyakov V.Y.
- Журнал: Biochemical and Biophysical Research Communications
- Том: 193
- Номер: 2
- Год издания: 1993
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 591
- Последняя страница: 598
- DOI: 10.1006/bbrc.1993.1665
- Аннотация: Treatment of the isolated nuclei with polyanions (PA) (heparin and dextransulfate) at the PA/DNA ratio of about 1 in the 0.15-0.5 M ammonium acetate solution leads to rearrangement of the chromatin structure and formation of compact globular particles (GP) 40-70 nm in diameter, bound by fibrils of variable thickness (2-10 nm). GP formation is accompanied by a loss of nucleosomal periodicity in DNA organization. However, no considerable loss of the DNA contents has been found. At the PA/DNA ratio of more than 2, destruction of the DNP-network and aggregation of GP in larger globular structures are observed. The same effect is observed under the nuclease treatment. Under this condition nuclei lose a considerable part of DNA, but retain histones supposedly as histone-PA aggregates. Therefore the joint effect of PA and salt on nuclei at certain conditions may lead to formation of artefact structures.
- Добавил в систему: Прусов Андрей Николаевич