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Zatsepina O. Development of novel mouse hybridomas producing monoclonal antibodies to human and mouse nucleolar protein SURF-6статья Электронная публикация
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 февраля 2017 г.
- Авторы: Polzikov M., Kordyukova M., Zavalishina L., Magoulas C.
- Журнал: Hybridoma
- Том: 31
- Номер: 1
- Год издания: 2012
- Издательство: Mary Ann Liebert Inc.
- Местоположение издательства: United States
- Первая страница: 48
- Последняя страница: 53
- Аннотация: SURF-6 is an evolutionarily conserved nucleolar protein that is important for cell viability; however, its function in mammals still remains uncertain. The aim of this study is to generate monoclonal antibodies to human SURF-6 protein suitable for fundamental and biomedical research. The full-size human SURF-6 was expressed as a recombinant GST-fusion protein and used as an antigen to generate monoclonal antibodies, S79 and S148, specific for SURF-6. The monoclonal antibody produced by hybridoma clone S79 specifically recognizes endogenous SURF-6 by Western and immunofluorescence analyses in various cultured human cells, and by immunohistochemistry in paraffin-embedded sections of human breast cancer samples. Moreover, S79 immunoprecipitates protein complexes containing SURF-6 from HeLa cells extracts. The antibody S79 recognizes SURF-6 only in human cells; however, the antibody produced by hybridoma clone S148 can detect SURF-6 of human and mouse origin. Monoclonal antibodies to the nucleolar protein SURF-6 described in this work can be a useful tool for studies of ribosome biogenesis in normal and cancer cells. DOI: 10.1089/hyb.2011.0078
- Добавил в систему: Зацепина Ольга Владимировна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Polzikov_Hybridoma_2012.pdf | 304,8 КБ | 22 февраля 2017 [zatsepina_olga] | |
| 2. | Полный текст | Polzikov_Hybridoma_2012_F40jio2.pdf | 304,8 КБ | 22 февраля 2017 [zatsepina_olga] |