Iron availability enhances the cellular energetics of aerobic Escherichia coli cultures while upregulating anaerobic respiratory chains

Baez, A.; Sharma, A.K.; Bryukhanov, A.; Anderson, E.D.; Olivares-Hernández, R.; Quan, D.; Shiloach, J.

Авторы: Baez Antonino, Sharma Ashish K., Bryukhanov Andrey, Anderson Eric D., Olivares-Hernández Roberto, Quan David, Shiloach Joseph
Журнал: New Biotechnology
Том: 71
Номер: 46
Год издания: 2022
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 11
Последняя страница: 20
DOI: 10.1016/j.nbt.2022.06.004
Аннотация: Aerobic Escherichia coli growth at restricted iron concentrations (≤ 1.75 ± 0.04 μM) is characterized by lower biomass yield, higher acetate accumulation and higher activation of the siderophore iron-acquisition systems. Although iron homeostasis in E. coli has been studied intensively, previous studies focused only on understanding the regulation of the iron import systems and the iron-requiring enzymes. Here, the effect of iron availability on the energy metabolism of E. coli has been investigated. It was established that aerobic cultures growing under limiting iron conditions showed lower ATP yield per glucose, lower growth rate and lower TCA cycle activity and respiration, at the same time as increased glucose consumption, acetate and pyruvate accumulation, practically mimicking microaerobic growth. However, at excess iron, independent of oxygen availability, the cultures showed high cellular energetics (5.8 ATP/mol of glucose) by using pathways requiring iron-rich complex proteins found in the TCA cycle and respiratory chain. In conditions of iron excess, some iron-requiring terminal reductases of the respiratory chain, that were thought to function only under anaerobiosis, were used by the E. coli, when in aerobic conditions, to maintain high respiratory activity. This allowed it to produce more biomass and more reactive oxygen species that were controlled by the higher activity of the antioxidant defenses (SOD, peroxidase and catalase) and the iron-sulfur cluster repair systems.
Добавил в систему: Брюханов Андрей Леонидович

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Iron availability enhances the cellular energetics of aerobic Escherichia coli cultures while upregulating anaerobic respiratory chainsстатья Исследовательская статья

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