Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide techniqueстатья
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Дата последнего поиска статьи во внешних источниках: 2 мая 2014 г.
Аннотация:Telomeres are the nucleoprotein complexes that cap the linear chromosome ends. Telomerase is a
ribonucleoprotein that maintains telomere length in stem, embryonic and cancer cells. Somatic cells
don’t contain active telomerase and telomere function as mitotic clock and telomere length determines
the number of cell divisions. Telomerase RNA (TER) contains the template for telomere synthesis and
serves as a structural scaffold for holoenzyme assembly. We compared different oligonucleotide based
methods for telomerase RNA inhibition, such as antisense oligonucleotides, knockdown by transient
siRNA transfection and silencing by miRNA derived from short expressed RNA hairpin in HEK293 cells. All
of these methods were applied to different TER regions. Our results revealed that CR2/CR3 domain of TER
is accessible in vitro and in vivo and could serve as an optimal site for oligonucleotide-based telomerase
silencing.