DIVERGENCE OF REITERATED DNA-SEQUENCES IN ECHINODERMS .2. COMPARISON OF SEQUENCES WITH A LOW DEGREE OF INTRAGENOME DIVERGENCEстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 мая 2015 г.
Аннотация:The DNA-DNA hybridization method was used to estimate the value of intergenomic divergence of sequences of "middle" and thermostable fracions among representatives of three classes of Echinodermata (Echinoidea, Asteroidea and Holothurioidea), and also representatives ofanother phyla, ascidian Halocynthia aurantium. The results obtained were compared with analogous measurements carried out with a fraction of thermolabile reitering sequences. It was found that in each of the three fractions there are sequences that are inherent in DNA of all the species compared. The proportion of of these sequences in thermolabile fraction is larger than that in the other two. The degree of divergence of the common sequences were estimated by the temperature stability of hybrid duplexes. Comparison of the related species shows that it is higher for the sequences of the thermostable fraction, whereas the sequences that are inherent in distant species diverged more markedly in the thermolabile fraction. Part of the sequences in the thermostable fractionform hybrid duplexes with a high melting temperature, even if one of the DNAs is from distant species. No such sequencea were found in the thermplabile fraction. The results obtained are discussed from the point view of the functional role of of various groups reiterating sequences and of the significans these data for the comprehension of the evolutional history of species.