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Beta- and Gamma-Cytoplasmic Actins Are Required for Meiosis in Mouse Oocytesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Brockmann Celine, Huarte Joachim, Dugina Vera, Challet Ludivine, Rey Emmanuelle, Conne Beatrice, Swetloff Adam, Nef Serge, Chaponnier Christine, Vassalli Jean-Dominique
- Журнал: Biology of Reproduction
- Том: 85
- Номер: 5
- Год издания: 2011
- Издательство: Society for the Study of Reproduction
- Местоположение издательства: United States
- Первая страница: 1025
- Последняя страница: 1039
- DOI: 10.1095/biolreprod.111.091736
- Аннотация: In mammals, female meiosis consists of two asymmetric cell divisions, which generate a large haploid oocyte and two small polar bodies. Asymmetric partitioning of the cytoplasm results from migration of the meiotic spindle toward the cortex and requires actin filaments. However, the subcellular localization and the role of the existing two cytoplasmic actin (CYA) isoforms, beta and gamma, have not been characterized. We show that beta- and gamma-CYA are differentially distributed in the maturing oocyte from late metaphase I as well as in preimplantation embryos. Gamma-CYA is preferentially enriched in oocyte cortices and is absent from all cell-cell contact areas from metaphase II until the blastocyst stage. Beta-CYA is enriched in contractile structures, at cytokinesis, at cell-cell contacts, and around the forming blastocoel. Alteration of beta- or gamma-CYA function by isoform-specific antibody microinjection suggests that gamma-CYA holds a major and specific role in the establishment and/or maintenance of asymmetry in meiosis I and in the maintenance of overall cortical integrity. In contrast, beta- and gamma-CYA, together, appear to participate in the formation and the cortical anchorage of the second meiotic spindle in waiting for fertilization. Finally, differences in gamma-CYA expression are amongst the earliest markers of cell fate determination in development.
- Добавил в систему: Дугина Вера Борисовна