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Genetic Characterization of the Central Variable Region in African Swine Fever Virus Isolates in the Russian Federation from 2013 to 2017статья
- Авторы: Mazloum A., Van Schalkwyk A., Chernyshev R., Shotin A., Korennoy F.I., Igolkin A., Sprygin A.
- Журнал: PATHOGENS
- Том: 11
- Номер: 919
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- DOI: 10.3390/pathogens11080919
- Аннотация: African swine fever virus (ASFV), classified as genotype II, was introduced into Georgiain 2007, and from there, it spread quickly and extensively across the Caucasus to Russia, Europeand Asia. The molecular epidemiology and evolution of these isolates are predominantly investi-gated by means of phylogenetic analysis based on complete genome sequences. Since this is a costlyand time-consuming endeavor, short genomic regions containing informative polymorphisms arepursued and utilized instead. In this study, sequences of the central variable region (CVR) locatedwithin the B602L gene were determined for 55 ASFV isolates submitted from 526 active Africanswine fever (ASF) outbreaks occurring in 23 different regions across the Russian Federation (RF)between 2013 and 2017. The new sequences were compared to previously published data availablefrom Genbank, representing isolates from Europe and Asia. The sequences clustered into six distinctgroups. Isolates from Estonia clustered into groups 3 and 4, whilst sequences from the RF weredivided into the remaining four groups. Two of these groups (5 and 6) exclusively contained isolatesfrom the RF, while group 2 included isolates from Russia as well as Chechnya, Georgia, Armenia,Azerbaijan and Ukraine. In contrast, group 1 was the largest, containing sequences from the RF,Europe and Asia, and was represented by the sequence from the first isolate in Georgia in 2007.Based on these results, it is recommended that the CVR sequences contain significant informativepolymorphisms to be used as a marker for investigating the epidemiology and spread of genotypeII ASFVs circulating in the RF, Europe and Asia
- Добавил в систему: Коренной Фёдор Игоревич