UTILIZING ARTERIES FROM ADULT HUMAN DONORS FOR BLOOD VESSELстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 21 июля 2017 г.
Аннотация:Introduction: Tissue engineered blood vessels promise to surpass vascular prostheses, auto- and allo- grafts in accessibility, size match, resistance against infection and thrombosis, mechanical compliance and endurance, non-immunogenicy, and physiological integration. We consider human acellular vascular matrix to be a strong candidate for clinical translation in midterm due to its properties either inherent or acquired by different means (mesenchymal stem cells (MSC), in particular). Material and Methods: Iliac arteries (n = 15) common, external and internal; small mesenteric arteries (n = 3) were taken from deceased heart beating donors during organ procurement. Before decellularization biomaterial had been transported in preservation solution at + 4C within 24 hours, stored at - 80C. Histological, DNA fluorescence and extraction, primary cytotoxicity tests were done. Results: The individual protocols of decellularization were developed for different arteries applying detergent-enzymatic method and simultaneous inside- and outside lumen perfusion, varying: the time of each step respectively to the inner diameter of vessels and wall thickness; and concentrations of detergents. Histological and fluorescence examination confirmed total cell elimination with the drop of DNA at least by 97%. Moreover the 3 layer structure of vessels, elastic membranes and lamellas, collagen fibers preserved their integrity. Primary cytotoxicity tests revealed that rabbit MSC are able to attach and successfully proliferate on the scaffold. Conclusion: We successfully developed process of decellularization of human arteries of various diameters with preservation of vessel's microarchitecture and cell-compatibility, which is important for graft size matching and maintaining long-term graft patency. This increases the potential of grafts for vascular reconstructive surgery usage.