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Oviductal Extracellular Vesicles Enhance Porcine In Vitro Embryo Development by Modulating the Embryonic Transcriptomeстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: de Alcântara-Neto Agostinho Soares, Cuello Cristina, Uzbekov Rustem, Bauersachs Stefan, Mermillod Pascal, Almiñana Carmen
- Журнал: Biomolecules
- Том: 12
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Номер статьи: 1300
- DOI: 10.3390/biom12091300
- Аннотация: Oviductal extracellular vesicles (oEVs) have been identified as important components ofthe oviductal fluid (OF) and have been pointed to as key modulators of gamete/embryo-maternalinteractions. Here, we determined the functional impact of oEVs on embryo development and theembryonic transcriptome in porcine. Experiment 1 examined the effect of oEVs and OF on embryodevelopment. In vitro-produced embryos were cultured with oEVs or OF for 2 or 7 days using anin vitro sequential system or without supplementation (control). Experiment 2 analyzed tran-scriptomic alterations of EV-treated embryos versus control and the oEVs RNA cargo by RNA-se-quencing. Two days of EV treatment enhanced embryo development over time when compared toother treatments. Different RNA expression profiles between embryos treated with EVs for two orseven days and untreated controls were obtained, with 54 and 59 differentially expressed (DE)genes and six and seven DE miRNAs, respectively. In oEV RNA cargo, 12,998 RNAs and 163 miR-NAs were identified. Integrative analyses pointed to specific oEV components that might act asmodulators of the embryonic transcriptome, such as S100A11, ANXA2 or miR-21-5p. Overall, thefindings suggested that oEVs could be a potential strategy to improve porcine IVP outcomes, par-ticularly by using two days of EV treatment.
- Добавил в систему: Узбеков Рустем Эдуардович