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INFLUENCE OF pH ON THE SPECTRAL-LUMINESCENT CHARACTERISTICS OF THE SOFT PROTEIN CROWN OF SILVER NANOPARTICLESстатья
- Авторы: Davidov F.D., Kuleshova A.A., Saletsky A.M.
- Журнал: Journal of Applied Spectroscopy
- Том: 89
- Номер: 2
- Год издания: 2022
- Издательство: Springer-Verlag
- Местоположение издательства: Heidelberg, Germany
- Первая страница: 281
- Последняя страница: 287
- DOI: 10.1007/s10812-022-01355-z
- Аннотация: The spectral and fl uorescent characteristics of bovine serum albumin (BSA) molecules in the soft crown of silver nanoparticles (Ag-NPs) were studied at various pH values. The formation of BSA + Ag-NP complexes was established. The association constants of the complex (Kas) and the bimolecular rate constants of the BSA fl uorescence quenching (Kq) were determined for various pH values. The dependences of Kas and Kq on pH were nonmonotonic with maxima at pH 6.0. The number of binding sites (Hill coeffi cient n) and the thickness of the BSA soft crown ( d) were also maximal at pH 6.0. These parameters decreased at higher and lower pH values (relative to pH 6.0). Variations in the interaction parameters of BSA and Ag-NPs were due to changes in the conformational modifi cations of the protein (content of α-helices) and the microenvironment of Tyr and Trp protein residues in the soft crown (hydrophobicity of the protein).
- Добавил в систему: Салецкий Александр Михайлович