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Cyclosporin A-sensitive cytochrome c release and activation of external pathway of NADH oxidation in liver mitochondria due to pore opening by acidification of phosphate-containing incubation mediumстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Knorre D.A., Dedukhova V.I., Vyssokikh M.Y., Mokhova E.N.
- Журнал: Bioscience Reports
- Том: 23
- Номер: 2-3
- Год издания: 2003
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 67
- Последняя страница: 75
- Аннотация: Acidification of a high phosphate incubation medium from pH 7.4 to 6.5 promotes increase in rates of succinate oxidation and exogenous NADH oxidation via external (rotenone- and myxothiazol-resistant) pathway by factors 2 and 2.3 respectively. Cyclosporin A prevents these effects. To measure the cytochrome c release, mitochondrial cytochrome c concentration was calculated from absorption spectrum of α-band of cytochromes c+c1. The cytochrome c release is shown to be equal to 27±4%, 40±12%, 70±5% at pH 7.4, 7.0, 6.5, respectively, the last value being reduced by cyclosporin A to 10±3%. Immunoblot method gives the similar results. It is concluded that acidification of the high phosphate medium induces release of a large part of the cytochrome c pool from liver mitochondria due to opening the Ca2+-dependent cyclosporin A-sensitive permeability transition pore and subsequent high amplitude swelling.
- Добавил в систему: Кнорре Дмитрий Алексеевич