Аннотация:The article highlights the results of the Lebedin cattle genetic structure evaluation by 12 microsatellite loci, kappa-casein and growth hormone genes polymorphism studies. Seventy allelic variants of microsatellite loci were identified and the largest number of alleles was observed for TGLA53 locus (10 alleles). All microsatellite DNA markers, except BM1818, showed a high degree of polymorphism. QTL gene allelic variants association with the cattle productive traits was investigated. A sufficiently high B-allele frequency (0.323) of kappa-casein gene was detected and a large number of animals (0.645) have the heterozygous AB genotype variant. The L-allele vari-ant predominance of the growth hormone gene was fixed (0.903). The complex genotypes affect-ing the value of such traits as milk yield, milk fat and protein content were identified.