Аннотация:This article presents the results of a study on 10 microsatellite DNA loci polymorphism in Charolais cattle (n=101). Seventy-seven allelic variants were identified and the largest number of alleles was observed for TGLA53 locus (12 alleles). All microsatellite DNA markers, except ETH10, showed a high degree of polymorphism. A significantly high B allele frequency (0.269) of kappa-casein gene was detected and a large number of animals (0.508) have the heterozygous AB genotype variant. The frequency of V allele (0.511) of the growth hormone gene was non-significantly higher than that of L allele (0.489). Animals with heterozygous LV genotype prevailed (0.537); frequencies of homozygous LL and VV genotypes were 0.221 and 0.242, respectively.