Generation of Monoclonal Antibody Against trans-Resveratrolстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 марта 2017 г.
Аннотация:Monoclonal antibody (MAb) against trans-resveratrol (t-RSV) was obtained from hybridoma clones constructed
from splenocytes of BALB/c mice immunized with carrier proteins (bovine serum albumin [BSA] and ovalbumin
[OVA]) coupled with synthetic hapten mimicking t-RSV structure. The t-RSV-BSA derivate was more
efficient at induction of the immune response than t-RSV-OVA. However, the use of t-RSV-OVA was advantageous
during selection of hybridoma clones constructed from splenocytes of t-RSV-BSA-immunized mice.
Pre-incubation of immune serum with free t-RSV inhibited the binding of antibody to t-RSV-BSA conjugate,
suggesting the specific nature of antibody binding to t-RSV. Splenocytes obtained from the mouse immunized
with t-RSV-BSA were used for the hybridoma construction. Expansion of the primary clones, their subsequent
screening, and subcloning narrowed our search to allowed isolation of two IgG1a-producing hybridomas
designated as 2H9 and 1B1. According to an indirect ELISA assay, the resulting MAb 2H9 had little or no crossreactivity
to cis-RSV. No recognition of trans-RSV-3-O-glucuronide and trans-RSV-3-sulfate was detected. The
newly generated MAb against t-RSV may provide a highly valuable and cost-effective tool for the analytical
assay for t-RSV in different biological and agricultural specimens.