Use of anchor protein modules in fluorescence polarisation aptamer assay for ochratoxin A determination

Samokhvalov, A.V.; Safenkova, I.V.; Eremin, S.A.; Zherdev, A.V.; Dzantiev, B.B.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 5 июня 2017 г.

Авторы: Samokhvalov Alexey V., Safenkova Irina V., Eremin Sergei A., Zherdev Anatoly V., Dzantiev Boris B.
Журнал: Analytica Chimica Acta
Том: 962
Год издания: 2017
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 80
Последняя страница: 87
DOI: 10.1016/j.aca.2017.01.024
Аннотация: A new strategy for sensitive fl uorescence polarisation (FP) analysis is proposed which uses aptamer as the receptor and anchor protein modules as the enhancers by including the aptamers in complexes with protein modules. This approach is based on increasing the size differences of bound and unbound fl u- orophores. The strategy was applied in an ochratoxin A ( ??? ) assay with the competitive binding of fl uorophore-labelled and free OTA with aptamer-based receptors. We showed that the binding of labelled OTA with aptamer included in complexes with anchors led to higher a FP than binding with free aptamer. This allowed the aptamer concentration to be reduced, thus lowering the limit of detection by a factor of 40, down to 3.6 nM. The assay time was 15 min. To evaluate the applicability of the FP assay with aptamer e anchor complex to real samples, we conducted OTA measurements in spiked white wine. The OTA limit of detection in wine was 2.8 nM (1.1 m g/kg), and the recoveries ranged from 83% to 113%. The study shows that the proposed anchor strategy is ef fi cient for increasing the sensitivity of FP-based aptamer assays
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Use of anchor protein modules in fluorescence polarisation aptamer assay for ochratoxin A determinationстатья

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