Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line

Baleva, M.V.; Chicherin, I.; Piunova, U.; Zgoda, V.; Patrushev, M.V.; Levitskii, S.; Kamenski, P.

Авторы: Baleva Maria V., Chicherin Ivan, Piunova Uliana, Zgoda Viktor, Patrushev Maxim V., Levitskii Sergey, Kamenski Piotr
Журнал: International Journal of Molecular Sciences
Том: 23
Номер: 22
Год издания: 2022
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Номер статьи: 14241
DOI: 10.3390/ijms232214241
Аннотация: Protein biosynthesis in mitochondria is tightly coupled with assembly of inner membrane complexes and therefore must be coordinated with cytosolic translation of the mRNAs corresponding to the subunits which are encoded in the nucleus. Molecular mechanisms underlying the regulation of mitochondrial translation remain unclear despite recent advances in structural biology. Until now, only one translational regulator of protein biosynthesis in mammalian mitochondria is known—protein TACO1, which regulates translation of COI mRNA. Here we describe the function of pentatricopeptide-containing protein PTCD2 as a translational regulator of another mitochondrially encoded subunit of cytochrome c oxidase—COIII in the HeLa cell line. Deletion of the PTCD2 gene leads to significant decrease in COIII translation efficiency and impairment in CIV activity. Additionally, we show that PTCD2 protein is partially co-sedimentates with associated mitochondrial ribosome and associates with mitochondrial ribosome proteins in pull-down assays. These data allow concluding that PTCD2 is a specific translational regulator of COIII which attracts the mRNA to the mitochondrial ribosome.
Добавил в систему: Каменский Петр Андреевич

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Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Lineстатья Исследовательская статья

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