|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Interaction between Nonhistone Protein HMGB1 and Linker Histone H1 Facilitates the Formation of Structurally Ordered DNA-Protein Complexesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 апреля 2017 г.
- Авторы: Polyanichko A., Chikhirzhina E.
- Журнал: Spectroscopy
- Том: 27
- Номер: 5-6
- Год издания: 2012
- Издательство: I O S press
- Местоположение издательства: Netherlands
- Первая страница: 393
- Последняя страница: 398
- DOI: 10.1155/2012/213031
- Аннотация: The structural organization of the DNA complexes with nonhistone chromosomal protein and linker histone H1 was studied using circular dichroism spectroscopy (CD) and atomic force microscopy (AFM). It has been shown that due to the interaction between HMGB1 and H1 highly ordered DNA-protein complexes emerge in the solution. Their spectral properties are found to be similar to those of DNA/HMGB1-(AB) complexes, reported earlier. AFM images reveal the formation of fibrillike structures in the solution. We suggest that the electrostatic screening of the HMGB1 C-terminal domain by histone H1 facilitates stronger interaction of the HMGB1/H1 with DNA and the formation of the ordered supramolecular DNA-protein complexes.
- Добавил в систему: Чихиржина Елена Всеволодовна