Interaction between DNA and chromosomal proteins HMGB1 and H1 studied by IR/VCD spectroscopyстатья
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Дата последнего поиска статьи во внешних источниках: 4 апреля 2017 г.
Аннотация:Binary complexes of calf thymus DNA with calf thymus non-histone chromosomal protein HMGB1 and
linker histone H1 were studied using FTIR/VCD spectroscopy. The spectroscopic data showed that the
interaction of the protein HMGB1 and histone H1 with DNA resulted in formation of two different types
of the macromolecular complexes. Histone H1 retained its native structure even at high concentrations
and induced DNA condensation upon binding at the protein to DNA ratio r (w/w) in the complex
rP0.3. HMGB1 demonstrated the ability to form soluble complexes at considerably higher protein to
DNA ratios. The obtained data suggest that the HMGB1 also participated in the protein–protein interactionsvia its C-terminal domain.