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System of Markers Based on the Methylation of a Group of Proapoptotic Genes in Combination with MicroRNA in the Diagnosis of Breast Cancerстатья
- Авторы: Braga EA, Burdennyy AM, Pronina IV, Filippova EA, Kazubskaya TP, Fridman MV, Khodyrev DS, Karpukhin AV, Loginov VI, Kushlinskii NE
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 168
- Номер: 3
- Год издания: 2020
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 366
- Последняя страница: 370
- DOI: 10.1007/s10517-020-04710-2
- Аннотация: Systems of markers for the diagnosis of breast cancer based on DNA methylation of a group of suppressor protein-coding genes, hypermethylated microRNA genes, and their combinations were compiled. On a representative sample of 70 paired breast cancer specimens (tumor/normal), MS-PCR analysis revealed a significant increase in the methylation frequency of 5 protein-coding genes: RASSF1A suppressor and apoptosis genes APAF1, BAX, BIM/BCL2L11, and DAPK1 (34-61% vs. 4-24%) and 6 microRNA genes: MIRG124G1, MIRG125bG1, MIRG129G2, MIRG148a, MIRG34b/c, and MIRG9G3 (36-76% vs. 6-27%). ROC-analysis showed that a combination of 4 genes (APAF1, BAX, BIM/BCL2L11, and DAPK1) and MIRG125bG1 gene constitute a highly efficient 5-marker system with 100% specificity and sensitivity of 94-96% at AUC=0.98-0.97, suitable also for patients with stage I and II breast cancer. Detection of methylation of at least one gene in this system in biopsy or postoperative material is sufficient to refer the sample to breast cancer.
- Добавил в систему: Казубская Татьяна Павловна