|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
The Store-operated Calcium Entry Pathways in Human Carcinoma A431 Cellsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Дата последнего поиска статьи во внешних источниках: 5 апреля 2017 г.
- Авторы: Gusev Konstantin, Glouchankova Lyuba, Zubov Alexander, Kaznacheyeva Elena, Wang Zhengnan, Bezprozvanny Ilya, Mozhayeva Galina N.
- Журнал: The Journal of General Physiology
- Том: 122
- Номер: 1
- Год издания: 2003
- Первая страница: 81
- Последняя страница: 94
- DOI: 10.1085/jgp.200308815
- Аннотация: Activation of phospholipase C (PLC)-mediated signaling pathways in nonexcitable cells causes the release of Ca2+ from intracellular Ca2+ stores and activation of Ca2+ influx across the plasma membrane. Two types of Ca2+ channels, highly Ca2+–selective ICRAC and moderately Ca2+–selective ISOC, support store-operated Ca2+ entry process. In previous patch-clamp experiments with a human carcinoma A431 cell line we described store-operated Imin/ICRACL plasma membrane Ca2+ influx channels. In the present paper we use whole-cell and single-channel recordings to further characterize store-operated Ca2+ influx pathways in A431 cells. We discovered that (a) ICRAC and ISOC are present in A431 cells; (b) ICRAC currents are highly selective for divalent cations and fully activate within 150 s after initiation of Ca2+ store depletion; (c) ISOC currents are moderately selective for divalent cations (PBa/PCs = 14.5) and require at least 300 s for full activation; (d) ICRAC and ISOC currents are activated by PLC-coupled receptor agonists; (e) ISOC currents are supported by Imin/ICRACL channels that display 8.5–10 pS conductance for sodium; (f) ICRAC single channel conductance for sodium is estimated at 0.9 pS by the noise analysis; (g) Imin/ICRACL channels are activated in excised patches by an amino-terminal fragment of InsP3R1 (InsP3R1N); and (h) InsP3 binding to InsP3R1N is necessary for activation of Imin/ICRACL channels. Our findings provide novel information about store-operated Ca2+ influx pathways in A431 cells.
- Добавил в систему: Гусев Константин Олегович