Complex of Proline-Specific Peptidases in the Genome and Gut Transcriptomes of Tenebrionidae Insects and Their Role in Gliadin Hydrolysis

Tereshchenkova, V.F.; Filippova, I.Y.; Goptar, I.A.; Dunaevsky, Y.E.; Belozersky, M.A.; Elpidina, E.N.

Авторы: Tereshchenkova V.F., Filippova I.Y., Goptar I.A., Dunaevsky Y.E., Belozersky M.A., Elpidina E.N.
Журнал: International Journal of Molecular Sciences
Том: 24
Номер: 1
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Номер статьи: 579
DOI: 10.3390/ijms24010579
Аннотация: A detailed analysis of the complexes of proline-specific peptidases (PSPs) in the midgut transcriptomes of the larvae of agricultural pests Tenebrio molitor and Tribolium castaneum and in the genome of T. castaneum is presented. Analysis of the T. castaneum genome revealed 13 PSP sequences from the clans of serine and metal-dependent peptidases, of which 11 sequences were also found in the gut transcriptomes of both tenebrionid species’ larvae. Studies of the localization of PSPs, evaluation of the expression level of their genes in gut transcriptomes, and prediction of the presence of signal peptides determining secretory pathways made it possible to propose a set of peptidases that can directly participate in the hydrolysis of food proteins in the larvae guts. The discovered digestive PSPs of tenebrionids in combination with the post-glutamine cleaving cysteine cathepsins of these insects effectively hydrolyzed gliadins, which are the natural food substrates of the studied pests. Based on the data obtained, a hypothetical scheme for the complete hydrolysis of immunogenic gliadin peptides by T. molitor and T. castaneum digestive peptidases was proposed. These results show promise regarding the development of a drug based on tenebrionid digestive enzymes for the enzymatic therapy of celiac disease and gluten intolerance.Keywords: proline-specific peptidases; dipeptidyl peptidase 4; transcriptomic analysis; Tenebrionidae insects; gliadin hydrolysisThis research was supported by the Russian Science Foundation (project № 22-24-00553).
Добавил в систему: Терещенкова Валерия Феликсовна

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Complex of Proline-Specific Peptidases in the Genome and Gut Transcriptomes of Tenebrionidae Insects and Their Role in Gliadin Hydrolysisстатья Исследовательская статья

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