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Two-stage implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts induced into osteoblast differentiation for direct skeletal attachment of limb prosthesesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 11 апреля 2017 г.
- Авторы: Shevtsov M.A., Galibin O.V., Yudintceva N.M., Blinova M.I., Pinaev G.P., Ivanova A.A., Savchenko O.N., Suslov D.N., Potokin I.L., Pitkin E., Raykhtsaum G., Pitkin M.R.
- Журнал: Journal of Biomedical Materials Research - Part A
- Том: 102
- Номер: 9
- Год издания: 2014
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 3033
- Последняя страница: 3048
- DOI: 10.1002/jbm.a.34969
- Аннотация: Angio- and osteogenesis following the two-stage (TS) implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts was evaluated. Two consecutive animal substudies were undertaken: intramedullary subcutaneous implantation (15 rabbits) and a TS transcutaneous implantation (12 rabbits). We observed enhanced osseointegrative properties of the intramedullary porous component seeded with fibroblasts induced into osteoblast differentiation, as compared to the untreated porous titanium pylon. The three-phase scintigraphy and subsequent histological analysis showed that the level of osteogenesis was 1.5-fold higher than in the control group, and significantly so (p < 0.05). The biocompatibility was further proved by the absence of inflammatory response or encapsulation and sequestration on the histology assay. Treatment of the transcutaneous component with autologous fibroblasts was associated with nearly a 2-fold decrease in the period required for the ingrowth of dermal and subdermal soft tissues into the implant surface, as compared to the untreated porous titanium component. Direct dermal attachment to the transcutaneous implant prevented superficial and deep periprosthetic infections in rabbits in vivo.
- Добавил в систему: Юдинцева Наталия Михайловна