Местоположение издательства:Hagerstown, MD, United States
Первая страница:332
Последняя страница:336
Аннотация:The discussion about the clinical significance of Blastocystis spp. and
its pathogenic potential is still ongoing. In recent decades, high rates
of blastocyst insemination have been identified in various population
groups. It is known that, being in the intestine, blastocysts participate
in the formation of the microbiome of the ecotope. Disrupting the
balance of microorganisms, these pathogens contribute to the
creation of favorable conditions for the development of pathological
processes. However, the mechanisms that reduce the protective
forces of the macroorganism and contribute to the development and
flow of the epidemiological process require deeper knowledge in
addressing this issue. In the presented work a relatively simple way
of determining a blastocyst with different degree of virulence using
modern restriction analysis of the protozoa genome has been
proposed. In the genome of blastocyst, a genetically determined
number of recognition sites for a certain type of restriction enzymes was found, which led to the formation of a strictly defined
number of DNA fragments of a fixed size in the study groups of
protozoans. The proposed combination of endonucleases (Hae III, Pst
I and Hind III) allowed to carry out species identification of strains of
Blastocystis spp. differing in virulence indices, and also to judge about
presence of different phenotypic groups of the protozoans in the
studied biotope