Establishment and Characterization of Clonal Lines with Cancer Stem- and Progenitor-Cell Properties from Monolayer Zajdela Hepatomaстатья
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Дата последнего поиска статьи во внешних источниках: 12 июля 2019 г.
Аннотация:Abstract. The aim of this study was to investigate the biology of cancer stem cells (CSC) from a metastatic
tumor. Previously, we explanted the cells of rat ascites Zajdela hepatoma in vitro. We established a permanent
monolayer cell line via selection of adhesive cells from multicellular floating islets. In the present work, we
cloned these cells by a limiting-dilution method and established five novel clonal sublines of the hepatoma:
three holoclonal sublines containing CSC and two meroclonal sublines. After a long-term cultivation
(approximately 30 passages, freezing, and thawing), the cell of clonal sublines retained the features of CSC.
They have a tumor-initiating potential and produce mainly holoclones upon recloning in the complete
growth medium and large nonadhesive hepatospheres in the serum-free medium. Morphometric analysis
showed that the cells of holoclonal and meroclonal sublines differed in the cell shape, area, nucleus size, and
nuclear-cytoplasmic ratio. We have found for the first time that holoclonal cells of Zajdela hepatoma have a
fibroblast-like morphology and form contacts with each other due to membrane protrusions. We suggest that
the fibroblast-like morphology of CSC is an attribute of a metastatic tumor and demonstrates the capability
of these cells for individual migration.