|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Enantioseparation of Beta-blockers Using Silica-Immobilised Eremomycin Derivatives as Chiral Stationary Phases in HPLCстатья Исследовательская статья
- Авторы: Kuznetsov Mikhail A., Staroverov Sergey M., Sarvin Nikita, Puzankov Ruslan, Nesterenko Pavel N.
- Журнал: Symmetry
- Том: 15
- Год издания: 2023
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Номер статьи: 373
- DOI: 10.3390/sym15020373
- Аннотация: The regularities of chromatographic retention and separation enantioselectivity of the selected -blockers (propranolol, pindolol, alprenolol, atenolol, oxprenalol, metoprolol, clenbuterol,sotalol, pronethalol, salbutamol, and labetalol) were studied with eight chiral stationary phases (CSPs) in polar ionic mode (PIM) elution system. A range of novel CSPs was prepared by immobilisation of macrocyclic glycopeptide antibiotic eremomycin (E-CSP); structurally related antibiotics chloreremomycin (Chloro-E-CSP) and semi-synthetic oritavancin (O-CSP); and five eremomycin derivatives including amide- (Amide-E-CSP), adamantyl-2-amide- (Adamantylamide-E-CSP), eremomycin aglycon (EAg-CSP), eremosaminyl eremomycin aglycon (EEA-CSP), and des-eremosamynyl eremomycin (DEE-CSP) onto microspherical silica (Kromasil, particle size 5 micron, pore size 11 nm). The effect of different functional groups in eremomycin structure on chiral recognition of -blockers was studied. The original E-CSP revealed moderate enantioseparation for all studied -blockers. The presence of a free carboxylic group in a chiral selector molecule is found to be critical for the general retention of enantiomers as no separation enantioselectivity was recorded for Amide-E-CSP and Adamantyl-E-CSP. Modification of the aromatic system of eremomycin by the introduction of a chloro- substituent in the aromatic ring (Chloro-E-CSP) or a hydrophobic 4’-chlorobiphenylmethyl substituent to the disaccharide sugar residue (O-CSP) resulted in decreased enantioselectivity. The best enantioseparation of -blockers was obtained for CSPs with eremosaminyl eremomycin aglyconand des-eremosamynyl eremomycin as chiral selectors.
- Добавил в систему: Нестеренко Павел Николаевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Статья | s15_00373_Kuznetsov.pdf | 5,3 МБ | 30 января 2023 [NesnterenkoPN] |