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Water-soluble N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan chloride as a nucleic acids vector for cell transfectionстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Faizuloev E.B., Marova A.A., Nikonova A.A., Volkova I.F., Gorshkova M.Yu, Izumrudov V.A.
- Журнал: Carbohydrate Polymers
- Том: 89
- Год издания: 2012
- Издательство: Pergamon Press Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 1088
- Последняя страница: 1094
- Аннотация: To endow the cationic polysaccharide with solubility in the whole pH-range without loss of functionality of the amino group, different chitosan samples were treated with glycidyltrimethylammonium chloride. Each modified unit of the exhaustively alkylated quaternized chitosan (QCht) consisted of both quaternary and secondary amino groups. The intercalated dye displacement assay and ζ-potential measurements implied stability of QCht polyplexes at physiological conditions and protonation of the secondary amino groups in slightly acidic media which is favorable for transfection, specifically according to proton sponge mechanism. The cytotoxicity and transfection efficacy of QCht increased with the chain lengthening, but relative to PEI, even highly polymerized samples were less toxic for COS-1 cells and revealed the comparable and significantly higher efficiency of transfection of siRNA and plasmid DNA, respectively. The established oppositely directed influence of QCht molecular weight on toxicity and transfection efficacy allows elaborating polysaccharide vectors with rational balance of these characteristics.
- Добавил в систему: Изумрудов Владимир Алексеевич