SERS uncovers the link between conformation of cytochrome c heme and mitochondrial membrane potential

Brazhe, N.A.; Nikelshparg, E.I.; Baizhumanov, A.A.; Grivennikova, V.G.; Semenova, A.A.; Novikov, S.M.; Volkov, V.S.; Arsenin, A.V.; Yakubovsky, D.I.; Evlyukhin, A.B.; Bochkova, Z.V.; Goodilin, E.A.; Maksimov, G.V.; Sosnovtseva, O.; Rubin, A.B.

Авторы: Brazhe Nadezda A., Nikelshparg Evelina I., Baizhumanov Adil A., Grivennikova Vera G., Semenova Anna A., Novikov Sergey M., Volkov Valentyn S., Arsenin Aleksey V., Yakubovsky Dmitry I., Evlyukhin Andrey B., Bochkova Zhanna V., Goodilin Eugene A., Maksimov Georgy V., Sosnovtseva Olga, Rubin Andrey B.
Журнал: Free Radical Biology and Medicine
Том: 196
Год издания: 2023
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 133
Последняя страница: 144
DOI: 10.1016/j.freeradbiomed.2023.01.013
Аннотация: The balance between the mitochondrial respiratory chain activity and the cell's needs in ATP ensures optimal cellular function. Cytochrome c is an essential component of the electron transport chain (ETC), which regulates ETC activity, oxygen consumption, ATP synthesis and can initiate apoptosis. The impact of conformational changes in cytochrome c on its function is not understood for the lack of access to these changes in intact mitochondria. We have developed a novel sensor that uses unique properties of label-free surface-enhanced Raman spectroscopy (SERS) to identify conformational changes in heme of cytochrome c and to elucidate their role in functioning mitochondria. We have verified that molecule bond vibrations assessed by SERS are a reliable indicator of the heme conformation during changes in the inner mitochondrial membrane potential and ETC activity. We have demonstrated that cytochrome c heme reversibly switches between planar and ruffled conformations in response to the inner mitochondrial membrane potential (ΔΨ) and H+ concentration in the intermembrane space. This regulates the efficiency of the mitochondrial respiratory chain, thus, adjusting the mitochondrial respiration to the cell's consumption of ATP and the overall activity. We have found that under hypertensive conditions cytochrome c heme loses its sensitivity to ΔΨ that can affect the regulation of ETC activity. The ability of the proposed SERS-based sensor to track mitochondrial function opens broad perspectives in cell bioenergetics.
Добавил в систему: Браже Надежда Александровна

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИПМех РАН

SERS uncovers the link between conformation of cytochrome c heme and mitochondrial membrane potentialстатья Исследовательская статья

Прикрепленные файлы