Structural perturbation of super-folder GFP in the presence of guanidine thiocyanate

Авторы: Stepanenko Olesya V., Stepanenko Olga V., Kuznetsova Irina M., Verkhusha Vladislav V., Turoverov Konstantin K.
Сборник: Volume 7: Spectroscopy of Biological Molecules
Серия: Advances in Biomedical Spectroscopy
Год издания: 2013
Первая страница: 249
Последняя страница: 254
DOI: 10.3233/978-1-61499-184-7-249
Аннотация: The guanidine thiocyanate induced denaturation-renaturation of sfGFP was studied. It was shown that the disruption of sfGFP native structure occurs in the range of guanidine thiocyanate concentrations from 0.5 to 2.5 M. This process was accompanied by simultaneous changes of all recorded parameters. It was found that the small guanidine thiocyanate concentrations (less then 0.1–0.2 M) triggered local structural disturbances in protein which result in significant decrease of chromophore and tryptophan fluorescence intensity and change of protein visible absorption spectrum.
Добавил в систему: Степаненко Ольга Викторовна

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