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Competitive Reactions in Solutions of the Complex of Modified Chitosan and DNAстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Izumrudov V.A., Zhiryakova M.V.
- Журнал: Polymer Science, Series A
- Том: 53
- Номер: 6
- Год издания: 2011
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 441
- Последняя страница: 448
- DOI: 10.1134/S0965545X1106006X
- Аннотация: The interaction of water-soluble modified chitosan with a polymethacrylate anion and DNA in aqueous and water–salt solutions is studied via the methods of fluorescence quenching with the use of a pyrenyl-labeled polyacid and the intercalated dye ethidium bromide. After passage from neutral to weakly acidic solutions, the stability of the polysaccharide complex with the polymethacrylate anion significantly increases, but, in the case of the DNA-containing complex, stability remains almost invariable. In spite of a much higher stability of complexes against salt in weakly ionized poly(methacrylic acid), the equilibrium of the competitive binding of chitosan shifts toward complexation with DNA, a result that is indicative of the dominant role of electrostatic interactions in complex formation. The capability for sharp and reversible variation in the state of the system in weakly acidic media may become the starting point for development of biotechnological systems for separation of biological mixtures and creation of vectors for delivery of genetic material that are based on cationic biodegradable polysaccharides.
- Добавил в систему: Жирякова Марина Владимировна