Genetic Diversity Among Pseudomonad Strains Associated with Cereal Diseases in Russian Federationстатья Глава в книге

Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.

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[1] Genetic diversity among pseudomonad strains associated with cereal diseases in russian federation / E. V. Matveeva, A. N. Ignatov, V. K. Bobrova et al. // Pseudomonas syringae Pathovars and Related Pathogens - Identification, Epidemiology and Genomics. — Springer Science+Business Media B.V, 2008. — P. 337–345. Basal glume rot caused by Pseudomonas syringae pv. atrofaeiens (PSA), has emerged as a major bacterial disease of wheat, barley, and rye in Russia. Forty-nine suspect strains of PSA isolated from diseased cereal plants from different regions of Russia were tentatively identified by production of a fluorescent pigment and confirmed by pathogenicity tests on the host of origin. F.ach strain was then grouped according to LOPAT (/cvan, oxidase, potato rot, argininc dihydrolasc, and fobacco hypersensitivity) assays. Ten strains were assigned to LOPAT group la, 13 to group lb, 5 to group 2, 4 to group 3, and 16 to group 5. Strains of each group were then characterized by I6S-23S rRNA Intergcnic Transcribed Region (ITR) sequencing and fingerprinted by restriction fragment length polymorphism of ITR. and repetitive PCR using REP. ERIC, and BOX primers. A phylogenetictree constructed from ITR sequence data revealed two discrete dusters, designated syringae and fluorescens. ERIC-PCR did not work well. However, BOX PCR produced very useful differential genomic fingerprints. There was a high correlation between LOPAT group la and BOX PCR patterns. The remaining groups showed a low correlation to BOX PCR patterns and a high level of genetic diversity.

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