Pulsed field gel electrophoresis: theory, instruments and applications

Автор: Nasonova E.S.
Журнал: Cell and Tissue Biology
Том: 2
Номер: 6
Год издания: 2008
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 557
Последняя страница: 565
Аннотация: Pulsed field gel electrophoresis (PFGE) is a technique for the fractionation of high-molecular weight DNA ranging from 10 kb to 10 Mb by electrophoresis in agarose gel with an electric field that alternates (pulsates) in two directions. This technology plays a key role in modern genomics, as it allows manipulations with DNA of whole chromosomes or their large fragments. In this review, we discuss (1) the theory behind PFGE; (2) different instruments based on the principle of pulsed field, as well as their advantages and limitations; (3) factors affecting the DNA mobility in PFGE gel; and (4) practical applications of the technique. Key words: PFGE, DNA fractionation, chromosomal DNA. DOI: 10.1134/S1990519X08060011
Добавил в систему: Насонова Елена Станиславовна

№	Имя	Описание	Имя файла	Размер	Добавлен
1.	2008_Nassonova_BCT_PFGE_review.pdf		2008_Nassonova_BCT_PFGE_review.pdf	302,6 КБ	14 апреля 2017 [NassonovaES]

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА