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Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cellsстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 апреля 2017 г.
- Авторы: Maximov Anton V., Vedernikova Elena A., Hinssen Horst, Khaitlina Sofia Y., Negulyaev Yuri A.
- Журнал: FEBS Letters
- Том: 412
- Номер: 1
- Год издания: 1997
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 94
- Последняя страница: 96
- DOI: 10.1016/s0014-5793(97)00754-0
- Аннотация: With the use of the patch-clamp technique, physiological mechanisms of Na+ channel regulation involving submembranous actin rearrangements were examined in human myeloid leukemia K562 cells. We found that the actin-severing protein gelsolin applied to cytoplasmic surface of membrane fragments at a high level of [Ca2+](i) (1 mu M) increased drastically the activity of Na-selective channels of 12 pS unitary conductance. In the experiments on intact cells, the elevation of [Ca2+](i) using the ionophore 4Br-A23187 also resulted in Na+ channel activation. Addition of actin to the cytoplasmic surface of membrane patches reduced this activity to background level, likely due to actin polymerization. Our data imply that Ca-dependent modulations of the actin cytoskeleton may represent one of the general mechanisms of channel regulation and cell signalling.
- Добавил в систему: Негуляев Юрий Алексеевич