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Positioning of Chromosomes in Human Spermatozoa Is Determined by Ordered Centromere Arrangementстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Mudrak Olga S., Nazarov Igor B., Jones Estella L., Zalensky Andrei O.
- Журнал: PLoS ONE
- Том: 7
- Номер: 12
- Год издания: 2012
- Издательство: Public Library of Science
- Местоположение издательства: United States
- DOI: 10.1371/journal.pone.0052944
- Аннотация: The intranuclear positioning of chromosomes (CHRs) is a well-documented fact; however, mechanisms directing such ordering remain unclear. Unlike somatic cells, human spermatozoa contain distinct spatial markers and have asymmetric nuclei which make them a unique model for localizing CHR territories and matching peri-centromere domains. In this study, we established statistically preferential longitudinal and lateral positioning for eight CHRs. Both parameters demonstrated a correlation with the CHR gene densities but not with their sizes. Intranuclear non-random positioning of the CHRs was found to be driven by a specific linear order of centromeres physically interconnected in continuous arrays. In diploid spermatozoa, linear order of peri-centromeres was identical in two genome sets and essentially matched the arrangement established for haploid cells. We propose that the non-random longitudinal order of CHRs in human spermatozoa is generated during meiotic stages of spermatogenesis. The specific arrangement of sperm CHRs may serve as an epigenetic basis for differential transcription/replication and direct spatial CHR organization during early embryogenesis.
- Добавил в систему: Назаров Игорь Борисович