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Synthesis of Cy5-Labelled C5-Alkynyl-modified cytidine triphosphates via Sonogashira coupling for DNA labellingстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Kuznetsova Viktoriya E., Shershov Valeriy E., Guseinov Teimur O., Miftakhov Rinat A., Solyev Pavel N., Novikov Roman A., Levashova Anna I., Zasedatelev Alexander S., Lapa Sergey A., Chudinov Alexander V.
- Журнал: Bioorganic Chemistry
- Том: 131
- Год издания: 2023
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 106315
- DOI: 10.1016/j.bioorg.2022.106315
- Аннотация: New applications of palladium-catalyzed Sonogashira-type cross-coupling reaction between C5-halogenated 2′-deoxycytidine-5′-monophosphate and novel cyanine dyes with a terminal alkyne group have been developed. The present methodology allows to synthesize of fluorescently labeled C5-nucleoside triphosphates with different acetylene linkers between the fluorophore and pyrimidine base in good to excellent yields under mild reaction conditions. Modified 2′-deoxycytidine-5′-triphosphates were shown to be good substrates for DNA polymerases and were incorporated into the DNA by polymerase chain reaction.
- Добавил в систему: Сольев Павел Николаевич