Poly(3-hydroxybutyrate) 3D-Scaffold–Conduit for Guided Tissue Sprouting

Zharkova, I.I.; Volkov, A.V.; Muraev, A.A.; Makhina, T.K.; Voinova, V.V.; Ryabova, V.M.; Gazhva, Y.V.; Kashirina, A.S.; Kashina, A.V.; Bonartseva, G.A.; Zhuikov, V.A.; Shaitan, K.V.; Kirpichnikov, M.P.; Ivanov, S.Y.; Bonartsev, A.P.

Авторы: Zharkova Irina I., Volkov Aleksey V., Muraev Aleksandr A., Makhina Tatiana K., Voinova Vera V., Ryabova Valentina M., Gazhva Yulia V., Kashirina Alena S., Kashina Aleksandra V., Bonartseva Garina A., Zhuikov Vsevolod A., Shaitan Konstantin V., Kirpichnikov Mikhail P., Ivanov Sergey Yu, Bonartsev Anton P.
Журнал: International Journal of Molecular Sciences
Том: 24
Номер: 8
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 6965
DOI: 10.3390/ijms24086965
Аннотация: Scaffold biocompatibility remains an urgent problem in tissue engineering. An especiallyinteresting problem is guided cell intergrowth and tissue sprouting using a porous scaffold with aspecial design. Two types of structures were obtained from poly(3-hydroxybutyrate) (PHB) using asalt leaching technique. In flat scaffolds (scaffold-1), one side was more porous (pore size 100–300 μm),while the other side was smoother (pore size 10–50 μm). Such scaffolds are suitable for the in vitrocultivation of rat mesenchymal stem cells and 3T3 fibroblasts, and, upon subcutaneous implantationto older rats, they cause moderate inflammation and the formation of a fibrous capsule. Scaffold-2sare homogeneous volumetric hard sponges (pore size 30–300 μm) with more structured pores. Theywere suitable for the in vitro culturing of 3T3 fibroblasts. Scaffold-2s were used to manufacture aconduit from the PHB/PHBV tube with scaffold-2 as a filler. The subcutaneous implantation of suchconduits to older rats resulted in gradual soft connective tissue sprouting through the filler materialof the scaffold-2 without any visible inflammatory processes. Thus, scaffold-2 can be used as a guidefor connective tissue sprouting. The obtained data are advanced studies for reconstructive surgeryand tissue engineering application for the elderly patients.
Добавил в систему: Жаркова Ирина Игоревна

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Poly(3-hydroxybutyrate) 3D-Scaffold–Conduit for Guided Tissue Sproutingстатья Исследовательская статья

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