Аннотация:A combination of TLC-bioautography, MALDI-TOF-MS, and LC-MS/MS methods was used to identify flavonoids with anti-α-amylase activity in extracts of L. pratensis L. (herb), L. polyphillus L. (fruits), T. lanceolata R. Br. (herb), and S. japonica L. (buds). After TLC-autobiography assay, substances with anti-amylase activity were identified by MALDI-TOF-MS followed by confirmation of the result by LC-MS/MS. Results of the study revealed that the flavonoids apigenin, luteolin, formononetin, genistein, and kaempferol display marked anti-α-amylase activity. Formononetin showed the largest activity. Compared with LC-MS/MS, MALDI-TOF-MS is a quick and convenient method; results can be obtained within minutes; and only minor sample amounts are required which allows us to analyze mixtures of substances without preliminary separation. However, the inability to distinguish between isomers is the main limitation of the method. Practical Application: Reducing digestive α-amylase activity prevents a spike in blood glucose after a meal, allowing prevention or delay of the onset of type 2 diabetes symptoms. The method described may allow plant foods and functional foods to be evaluated for their ability to inhibit the digestive enzyme α-amylase. In addition, the results obtained on the anti-α-amylase activity of flavonoid aglycones can be used to develop nutritional supplements for the prevention of type 2 diabetesFirst published: 08 May 2023
