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Detection of the potyviral genome-linked protein VPg in virions and its phosphorylation by host kinasesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 12 февраля 2014 г.
- Авторы: Puustinen Pietri, Rajamäki Minna-Liisa, Ivanov Konstantin I., Valkonen Jari P.T., Mäkinen Kristiina
- Журнал: Journal of Virology
- Том: 76
- Номер: 24
- Год издания: 2002
- Издательство: American Society for Microbiology
- Местоположение издательства: United States
- Первая страница: 12703
- Последняя страница: 12711
- Аннотация: The multifunctional genome-linked protein (VPg) of Potato virus A (PVA; genus Potyvirus) was found to be phosphorylated as a part of the virus particle by a cellular kinase activity from tobacco. Immunoprecipitation, immunolabeling, and immunoelectron microscopy experiments showed that VPg is exposed at one end of the virion and it is accessible to protein-protein interactions. Substitution Ser185Leu at the C-proximal part of VPg reduces accumulation of PVA in inoculated leaves of the wild potato species Solanum commersonii and delays systemic infection, which is not observed in tobacco plants. Our data show that kinases of S. commersonii differentially recognize the VPg containing Ser or Leu at position 185, whereas both forms of VPg are similarly recognized by tobacco kinases. Taken together, our data imply that the virion-bound VPg may interact with host proteins and that phosphorylation of VPg may play a role in the VPg-mediated functions during the infection cycle of potyviruses.
- Добавил в систему: Иванов Константин Ильич