Аннотация:The assessment of anaerobic biotransformation in thermophilic mode (55 °C) of agricultural waste was carried out. It has been established that organic wastes (wheat distillery vinasse, cow manure and chicken manure) have a great potential for producing biogas with a high content of methane and carbon dioxide with a low content of impurities. Biogas can be recommended as a raw material for hydrogen production. With the ratio of components in the composition of the mixed substrate of distillery vinasse, cow manure and chicken manure - 6: 3: 1 for 21–29 days of anaerobic digestion, the best indicators of the efficiency of methanogenesis for methane (61–63%) and biogas (70–73%) were provided, the share of methane in composition of biogas was 61%. With the biotransformation of alone vinasse, the nitrogen mineralization efficiency (58–67%) was lower than when using a mixture of organic waste (63–69%). From the point of view of obtaining the maximum amount of biogas, it is advisable to add a carbonate buffer or limestone flour (9% wt.) to the substrate to shift the pH of the working solution towards slightly alkaline values. From the point of view of the accumulation of free phosphates in the final solution, the use of a carbonate buffer is more preferable. The anaerobically treated solid sludge remaining after biogas production, as well as liquid effluents (anaerobically treated effluents) can be considered as potential organic and biogenic fertilizers or their components.