|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Investigation of esterase status as a complex biomarker of exposure to organophosphorus compoundsстатья
- Авторы: Makhaeva G.F., Rudakova E.V., Sigolaeva L.V.
- Сборник: Toxicological Problems
- Глава в коллективной монографии
- Год издания: 2014
- Место издания: Military Publishing House Sofia, Bulgaria
- Первая страница: 15
- Последняя страница: 26
- Аннотация: Development of biomarkers of human exposures to OPCs and their quantification is a vital component of a system of prediction and early diagnosis of induced diseases. Our study was focused on investigation of esterase status as a complex biomarker of exposure to OPCs and an aid in accurate diagnosis. We suggest that this complex biomarker should be more effective and informative than standard assays of plasma BChE, RBC AChE, and lymphocyte neuropathy target esterase (NTE). It will allow us: 1) to assess an exposure as such and to confirm the nonexposure of individuals suspected to have been exposed; 2) to determine if the exposure was to agents expected to produce acute and/or delayed neurotoxicity; 3) to perform dosimetry of the exposure, which provides valuable information for medical treatment. To confirm this hypothesis, we examined the changes in activity of blood AChE, NTE, BChE and carboxylesterase (CaE) 1 h after i.p. administration of increasing doses of three OPCs with different esterase profiles: (C2H5O)2P(O)OCH(CF3)2, (C4H9O)2P(O)OCH(CF3)2 and (C3H7O)2P(O)OCH=CCl2. The esterases assay was performed in hemolysed blood by biosensor and spectrophotometric methods. Analysis of the obtained dose-dependences for blood esterases inhibition showed that blood BChE and CaE are the most sensitive biomarkers, allowing detection of low doses. Inhibition of blood NTE and AChE can be used to assess the likelihood that an exposure to OPC would produce cholinergic and/or delayed neuropathic effects. Thus, determination of esterase status allows one to improve the possibilities of diagnostics of exposure to OPCs.
- Добавил в систему: Сиголаева Лариса Викторовна