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Esterase status of various species in assessment of exposure to organophosphorus compoundsстатья
- Авторы: Boltneva N.P., Rudakova E.V., Sigolaeva L.V., Makhaeva G.F.
- Сборник: Toxicological Problems
- Глава в коллективной монографии
- Год издания: 2014
- Место издания: Military Publishing House Sofia, Bulgaria
- Первая страница: 27
- Последняя страница: 38
- Аннотация: Our project supposes to use an esterase status of an organism as a complex biomarker of exposure and sensitivity to organophosphorus compounds (OPCs). The esterase status includes five blood esterases: acetylcholinesterase (AChE), neuropathy target esterase (NTE), butyrylcholinesterase (BChE), carboxylesterase (CaE) and paraoxonase (PON1). The level of activity of these enzymes and their relationship to each other are an individual feature of the organism and are determined by its species, age, sex and genetic characteristics. Therefore, a set of esterases which are the most effective biomarkers in assessment of exposure to OPC may be different for various species. In this regard, it is important to determine the esterase status, because such an approach provides the most complete information on the character and extent of the exposure. In this study activities of esterases in the blood samples from humans and rodents (mice and rats) were measured by the spectrophotometric methods (AChE, BChE, CaE, PON1) or electrochemically (NTE). The obtained sets of the esterase activities were analyzed. The results indicate significant species differences in the esterase status: in humans cholinesterase activities are significant and CaE activity is negligible, while in rats and mice CaE activity is higher and cholinesterase activities are low. The rodents NTE activity is lower than that one in humans. Very high PON1 arylesterase activity has been found in all species.
- Добавил в систему: Сиголаева Лариса Викторовна