The use of thermal lensing for the determination of pyrogensстатья

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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.

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[1] The use of thermal lensing for the determination of pyrogens / N. V. Orlova, M. A. Proskurnin, V. A. Samburova et al. // Analytical and Bioanalytical Chemistry. — 2003. — Vol. 375, no. 8. — P. 1038–1044. Based on the optimized spectrophotometric determination of pyrogens (of various classes (p-aminophenol and endotoxins), thermal lensing was applied to the determination of these substances at the submicrogram level. The limit of detection of p-aminophenol, a pyrogenic impurity in pharmaceutical formulations of paracetamol, by reaction with resorcinol in alkaline solutions is 100 ng mL(-1). Phloroglucinol was considered as an analog of resorcinol as a reagent in this reaction. The conditions of spectrophotometric determination of pyrogenic lipopolysaccharides (endotoxins) by ion-pair formation with methylene blue (the limit of detection is 100 ng mL-1), by ion-pair formation with Stains-All (1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropeny l]naphtho[1,2-d]thiazolium bromide) (the limit of detection is 500 ng mL(-1)), and by reaction of 2-keto-3-deoxyoctonic acid with thiobarbituric acid (the limit of detection is 800 ng mL(-1)) were proposed. The optimized procedure for 2-keto-3-deoxyoctonic acid was applied for thermal lensing that provided a decrease in the limit of detection to 70 ng mL(-1) and was also used for lipopolysaccharide determination in the endotoxin standard from E. coli. [ DOI ]

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