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Association of 2‐oxoacid dehydrogenase complexes with respirasomes in mitochondriaстатья
- Авторы: Plokhikh Konstantin S., Nesterov Semen V., Chesnokov Yuriy M., Rogov Anton G., Kamyshinsky Roman A., Vasiliev Aleksandr L., Yaguzhinsky Lev S., Vasilov Raif G.
- Журнал: FEBS Journal
- Год издания: 2023
- Издательство: Blackwell Publishing Inc.
- Местоположение издательства: United Kingdom
- DOI: 10.1111/febs.16965
- Аннотация: In the present study, cryo-electron tomography was used to investigate the localization of 2-oxoacid dehydrogenase complexes (OADCs) in cardiac mitochondria and mitochondrial inner membrane samples. Two classes of ordered OADC inner cores with different symmetries were distinguished and their quaternary structures modeled. One class corresponds to pyruvate dehydrogenase complexes and the other to dehydrogenase complexes of α-ketoglutarate and branched-chain α-ketoacids. OADCs were shown to be localized in close proximity to membrane-embedded respirasomes, as observed both in densely packed lamellar cristae of cardiac mitochondria and in ruptured mitochondrial samples where the dense packing is absent. This suggests the specificity of the OADC–respirasome interaction, which allows localized NADH/NAD+ exchange between OADCs and complex I of the respiratory chain. The importance of this local coupling is based on OADCs being the link between respiration, glycolysis and amino acid metabolism. The coupling of these basic metabolic processes can vary in different tissues and conditions and may be involved in the development of various pathologies. The present study shows that this important and previously missing parameter of mitochondrial complex coupling can be successfully assessed using cryo-electron tomography.
- Добавил в систему: Нестеров Семён Валерьевич