FeIII–TAMLactivator: A potent peroxidase mimic for chemiluminescent determination of hydrogen peroxide

Vdovenko, M.M.; Demiyanova, A.S.; Kopylov, K.E.; Sakharov, I.Y.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 21 июля 2014 г.

Авторы: Vdovenko Marina M., Demiyanova Alexandra S., Kopylov Kirill E., Sakharov Ivan Yu
Журнал: Talanta
Том: 125
Год издания: 2014
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 361
Последняя страница: 365
DOI: 10.1016/j.talanta.2014.03.040
Аннотация: Efforts to replace native peroxidase with its low molecular weight alternatives have stimulated a search for peroxidase mimetics. Herein we describe the oxidation of luminol with hydrogen peroxide catalyzed by commercial available FeIII-TAML activator 1a, which was shown to be a more active catalyst than hemin. At FeIII-TAML activator 1a use in chemiluminescent assay for H2O2 determination the detection limit value (3σ) of 5 x 10-8 M was similar to the detection limit obtained with horseradish peroxidase (1 x 10-7 M) and significantly lower than that obtained in the presence of hemin (6 x 10-7 M). The linear ranges (R2 = 0.98) of the assay were 6 x 10-8 – 1 x 10-6 M and 6 x 10-7 – 1 x 10-6 M H2O2 for FeIII-TAML 1a and hemin, respectively. The CV values for FeIII-TAML 1a-based assay measured within the working range varied from 1.0 to 3.7 % (n=4), whereas in the case of hemin – 5.0 to 9.7 % (n=4). Moreover, the sensitivity of FeIII-TAML 1a-based method was 56 and 5 times higher than that of hemin- and HRP-based methods, respectively. The obtained results open good perspectives to apply FeIII-TAML activator 1a in CL analytical methods instead of hemin, a traditionally used peroxidase mimetic.
Добавил в систему: Сахаров Иван Юрьевич

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FeIII–TAMLactivator: A potent peroxidase mimic for chemiluminescent determination of hydrogen peroxideстатья

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