Investigation of the Mechanism of Membrane Potential Generation by Heme-Copper Respiratory Oxidases in a Real Time Mode

Siletsky, S.A.

Автор: Siletsky Sergei A.
Журнал: Biochemistry (Moscow)
Том: 88
Номер: 10
Год издания: 2023
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 1513
Последняя страница: 1527
Оригинальные версии: ИССЛЕДОВАНИЕ МЕХАНИЗМА ГЕНЕРАЦИИ МЕМБРАННОГО ПОТЕНЦИАЛА ГЕМ-МЕДНЫМИ ДЫХАТЕЛЬНЫМИ ОКСИДАЗАМИ В РЕЖИМЕ РЕАЛЬНОГО ВРЕМЕНИ
DOI: 10.1134/s0006297923100085
Аннотация: Heme-copper respiratory oxidases are highly efficient molecular machines. These membrane enzymes catalyze the final step of cellular respiration in eukaryotes and many prokaryotes: the transfer of electrons from cytochromes or quinols to molecular oxygen and oxygen reduction to water. The free energy released in this redox reaction is converted by heme-copper respiratory oxidases into the transmembrane gradient of the electrochemical potential of hydrogen ions ΔμH+). Heme-copper respiratory oxidases have a unique mechanism for generating ΔμH+, namely, a redox-coupled proton pump. A combination of direct electrometric method for measuring the kinetics of membrane potential generation with the methods of prestationary kinetics and site-directed mutagenesis in the studies of heme-copper oxidases allows to obtain a unique information on the translocation of protons inside the proteins in real time. The review summarizes the data of studies employing time-resolved electrometry to decipher the mechanisms of functioning of these important bioenergetic enzymes.
Добавил в систему: Силецкий Сергей Алексеевич

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИПМех РАН

Investigation of the Mechanism of Membrane Potential Generation by Heme-Copper Respiratory Oxidases in a Real Time Modeстатья

Прикрепленные файлы