Electrometric and Electron Paramagnetic Resonance Measurements of a Difference in the Transmembrane Electrochemical Potential: Photosynthetic Subcellular Structures and Isolated Pigment–Protein Complexesстатья
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Дата последнего поиска статьи во внешних источниках: 15 февраля 2024 г.
Аннотация:A transmembrane difference in the electrochemical potentials of protons (∆µH+) serves as a free energy intermediate in energy-transducing organelles of the living cell. The contributions of two components of the ∆µH+ (electrical, ∆ψ, and concentrational, ∆pH) to the overall ∆µH+ value depend on the nature and lipid composition of the energy-coupling membrane. In this review, we briefly consider several of the most common instrumental (electrometric and EPR) methods for numerical estimations of ∆ψ and ∆pH. In particular, the kinetics of the flash-induced electrometrical measurements of ∆ψ in bacterial chromatophores, isolated bacterial reaction centers, and Photosystems I and II of the oxygenic photosynthesis, as well as the use of pH-sensitive molecular indicators and kinetic data regarding pH-dependent electron transport in chloroplasts, have been reviewed. Further perspectives on the application of these methods to solve some fundamental and practical problems of membrane bioenergetics are discussed.