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Azithromycin Susceptibility Testing and Molecular Investigation of Neisseria gonorrhoeae, 2020-2021статья Электронная публикация
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Kandinov I., Shaskolskiy B., Kravtsov D., Vinokurova A., Gorshkova S., Kubanov A., Solomka V., Shagabieva J., Deryabin D., Dementieva E.
- Журнал: Antibiotics
- Том: 12
- Номер: 1
- Год издания: 2023
- Издательство: MDPI Publishing
- Местоположение издательства: Basel, Switzerland, Switzerland
- Номер статьи: 170
- DOI: 10.3390/antibiotics12010170
- Аннотация: The aim of this work was to study the resistance to macrolides (azithromycin) in the modern Russian population of N. gonorrhoeae with the analysis of genetic resistance determinants. Azithromycin is not used to treat gonococcal infection in Russia. However, among 162 isolates collected in 2020-2021, 22 isolates (13.6%) were phenotypically resistant to azithromycin. Mutations in 23S rRNA genes were found only in two isolates; erm and mefA genes were absent. Azithromycin resistance was shown to be predominantly associated with mutations in the mtrR and mtrD genes of the MtrCDE efflux pump and their mosaic alleles which may have formed due to a horizontal transfer from N. meningitidis. A total of 30 types of mtrR alleles and 10 types of mtrD alleles were identified including mosaic variants. Matching between the mtrR and mtrD alleles was revealed to indicate the cooperative molecular evolution of these genes. A link between the mtrR and mtrD alleles and NG-MAST types was found only for NG-MAST 228 and 807, typical of N. gonorrhoeae in Russia. The high level of resistance to azithromycin in Russia may be related to the spread of multiple transferable resistance to antimicrobials regardless of their use in the treatment of gonococcal infection.
- Добавил в систему: Винокурова Александра Сергеевна