Diversity and Functional Potential of Prokaryotic Communities in Depth Profile of Boreo‐Nemoral Minerotrophic Pine Swamp (European Russia)

Golovchenko, A.V.; Dobrovolskaya, T.G.; Sokolova, D.S.; Gracheva, T.A.; Dorchenkova, Y.A.; Glukhova, T.V.; Manucharova, N.A.

Авторы: Golovchenko Alla V., Dobrovolskaya Tatiana G., Sokolova Diyana Sh, Gracheva Tatiana A., Dorchenkova Yuliya A., Glukhova Tamara V., Manucharova Natalia A.
Журнал: FORESTS
Том: 14
Номер: 12
Год издания: 2023
Издательство: MDPI Publishing
Местоположение издательства: Basel, Switzerland, Switzerland
Первая страница: 2313
Последняя страница: 2338
DOI: 10.3390/f14122313
Аннотация: Natural peatlands represent a wide range of habitats that contribute to the conservationof biodiversity, including microbial biodiversity. Molecular biological methods make it possible tosignificantly increase the accounting of microbial diversity compared to the cultivation methods.The studies on microbial diversity in minerotrophic peatlands using molecular biological methodslag significantly behind such studies for ombrotrophic peatlands. In this work, we characterizedthe taxonomic composition and functional potential of the prokaryotic community of the minero‐trophic pine swamp (fen) in the Tver region of northwestern Russia using high‐throughput se‐quencing of 16S rRNA gene fragments. This study is unique, since it was carried out not in indi‐vidual horizons but across the entire fen profile, taking into account the differentiation of the pro‐file into the acrotelm and catotelm. The composition and dominants of bacterial and archaealcommunities were determined not only at the level of phyla but also at the level of classes, families,and cultivated genera. The prokaryotic community of the studied fen was shown to have a hightaxonomic diversity (28 bacterial and 10 archaeal phyla were identified). The profile differentiationof the taxonomic composition of prokaryotic communities is most clearly manifested in the analy‐sis of the acrotelm and catotelm. In the bacterial communities of the acrotelm, the top three phylaincluded Acidobacteriota, Alphaproteobacteria, and Actinomycetota, in the ca‐totelm—Betaproteobacteria, Bacteroidota, and Chloroflexota. In archaeal communities of theacrotelm, we discovered the monodominance of Nitrososphaerota, in the catotelm—the dominanceof Bathyarchaeota and subdominance of Thermoplasmatota, Halobacterota, and Aenigmarchaeota.The hot spots of microbial diversity in the studied fen profile were found to be the 0–20 cm layer ofthe acrotelm and the 150–200 cm layer of the catotelm. In contrast to the taxonomic composition,the functional profiles of the prokaryotic communities of the acrotelm and catotelm were generallysimilar, except for methane metabolism, which was primarily carried out in the catotelm.
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1.	Полный текст		forests-14-02313.pdf	987,7 КБ	24 ноября 2023 [N.A.Manucharova]
2.	Adobe_Scan_27_noyab._2023_g..pdf		Adobe_Scan_27_noyab._2023_g..pdf	412,7 КБ	27 ноября 2023 [GrachevaTA]

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Diversity and Functional Potential of Prokaryotic Communities in Depth Profile of Boreo‐Nemoral Minerotrophic Pine Swamp (European Russia)статья Исследовательская статья

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