Raman spectroscopy with near IR excitation for study of structural components of cyanobacterial phycobilisomes

Perevedentseva, E.; Melnik, N.; Muronets, E.; Averyushkin, A.; Karmenyan, A.; Elanskaya, I.

Авторы: Perevedentseva Elena, Melnik Nikolai, Muronets Elena, Averyushkin Anatoly, Karmenyan Artashes, Elanskaya Irina
Журнал: Journal of Luminescence
Том: 265
Год издания: 2024
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Номер статьи: 120224
DOI: 10.1016/j.jlumin.2023.120224
Аннотация: Cyanobacteria are prokaryotes that carry out oxygenic photosynthesis and are widely used as a model in scientific research. Being a rich source of biologically active compounds, they are also currently considered as a promising object for use in biotechnologies. For solving research or applied problems of cyanobacteria study,detection, identifying and monitoring of biotechnological production different kinds of laser spectroscopy can be a promising tools. In the present work Raman spectroscopy with IR excitation (IR Raman) is used to analyze phycobiliproteins, structural components of phycobilisomes – light-harvesting antenna – of the cyanobacterium Synechocystis sp. PCC6803. Excitation wavelengths in red/near infrared range were chosen to focus on thevibrational spectra and avoid an influence of pigments fluorescence.􀀀 The Raman spectra with excitation in near infrared and UV–visible absorption spectra were measured from wild type (WT) of Synechocystis sp. PCC6803, the mutant lacking C-phycocyanin (CK), and the mutant totally devoid of phycobilisomes (PAL). Spectral information is analyzed in comparison with data on structural and functional state of photosynthetic system of the cyanobacteria. It has been shown that IR Raman spectroscopy makes it possible to analyze in detail the structural disorders in phycobilisomes and to discuss the carotenoids-based mechanisms of compensation for this deficiency.
Добавил в систему: Еланская Ирина Владимировна

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Raman spectroscopy with near IR excitation for study of structural components of cyanobacterial phycobilisomesстатья

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