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Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSODстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 15 февраля 2024 г.
- Авторы: Cataldi Samuela, Borrelli Antonella, Ceccarini Maria Rachele, Nakashidze Irina, Codini Michela, Belov Oleg, Ivanov Alexander, Krasavin Eugene, Ferri Ivana, Conte Carmela, Patria Federica Filomena, Beccari Tommaso, Mancini Aldo, Curcio Francesco, Ambesi-Impiombato Francesco Saverio, Albi Elisabetta
- Журнал: International Journal of Molecular Sciences
- Том: 21
- Номер: 9
- Год издания: 2020
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Номер статьи: 3281
- DOI: 10.3390/ijms21093281
- Аннотация: Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due to Caspase-1 involvement. To study liver pyroptosis and hepatic SM metabolism via both lysosomal acid SMase (aSMase) and endoplasmic reticulum/nucleus neutral SMase (nSMase) during the exposure of mice to radiation and to ascertain if this process can be modulated by protective molecules, we used an experimental design (previously used by us) to evaluate the effects of both ionizing radiation and a specific protective molecule (rMnSOD) in the brain in collaboration with the Joint Institute for Nuclear Research, Dubna (Russia). As shown by the Caspase-1 immunostaining of the liver sections, the radiation resulted in the loss of the normal cell structure alongside a progressive and dose-dependent increase of the labelling, treatment, and pretreatment with rMnSOD, which had a significant protective effect on the livers. SM metabolic analyses, performed on aSMase and nSMase gene expression, as well as protein content and activity, proved that rMnSOD was able to significantly reduce radiation-induced damage by playing both a protective role via aSMase and a preventive role via nSMase.
- Добавил в систему: Белов Олег Валерьевич