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“3-in-1” Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollutionстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 15 февраля 2024 г.
- Авторы: Kamanina Olga A., Lantsova Elizaveta A., Rybochkin Pavel V., Arlyapov Vyacheslav A., Saverina Evgeniya A., Kulikovskaya Natalia S., Perepukhov Alexander M., Vereshchagin Anatoly N., Ananikov Valentine P.
- Журнал: ACS applied materials & interfaces
- Том: 15
- Номер: 40
- Год издания: 2023
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 47779
- Последняя страница: 47789
- DOI: 10.1021/acsami.3c09897
- Аннотация: This study presents a novel ″3-in-1″ hybrid biocatalyst design that combines the individual efficiency of microorganisms while avoiding negative interactions between them. Yeast cells of Ogataea polymorpha VKM Y-2559, Blastobotrys adeninivorans VKM Y-2677, and Debaryomyces hansenii VKM Y-2482 were immobilized in an organosilicon material by using the sol–gel method, resulting in a hybrid biocatalyst. The catalytic activity of the immobilized microorganism mixture was evaluated by employing it as the bioreceptor element of a biosensor. Optical and scanning electron microscopies were used to examine the morphology of the biohybrid material. Elemental distribution analysis confirmed the encapsulation of yeast cells in a matrix composed of methyltriethoxysilane (MTES) and tetraethoxysilane (TEOS) (85 and 15 vol %, respectively). The resulting heterogeneous biocatalyst exhibited excellent performance in determining the biochemical oxygen demand (BOD) index in real surface water samples, with a sensitivity coefficient of 50 ± 3 × 10–3·min–1, a concentration range of 0.3–31 mg/L, long-term stability for 25 days, and a relative standard deviation of 3.8%. These findings demonstrate the potential of the developed hybrid biocatalyst for effective pollution monitoring and wastewater treatment applications.
- Добавил в систему: Анаников Валентин Павлович